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AAV-FIB galanin Plasmid  AAV
An AAV2 ITR containing plasmid that has a Chicken Beta-Actin promoter (cBa promoter), a fibronectin secretory signal sequence, cDNA that codes for the active rat Galanin neuropeptide, and an SV-40 PolyA sequence. This plasmid causes the constitutive expression and secretion of mature rat galanin. 
 
AAV2.5 Plasmid  AAV
This plasmid is based on the XR2 plasmid, however 5 mutations were introduced into the 5 fold axis of symmetry.  These mutations allow vectors generated from this plasmid to have a greater tropism for muscle as opposed to its parental XR2 plasmid.  
 
AAV6.1-F129L Plasmid  AAV
AAV serotypes 1 and 6 share 99% homology in that only a 6 amino acid difference separates the two. Researchers were able to individually change these amino acids to represent the residue expressed by the other serotype and vice versa. The amino acids and their changes are listed below for this plasmid and include the parental residue, position of the residue, and residue changed to. AAV6.1-F129L 
 
AAV6.2-D418E Plasmid  AAV
AAV serotypes 1 and 6 share 99% homology in that only a 6 amino acid difference separates the two. Researchers were able to individually change these amino acids to represent the residue expressed by the other serotype and vice versa. The amino acids and their changes are listed below for this plasmid and include the parental residue, position of the residue, and residue changed to. AAV6.2-D418E 
 
AAV6.3-K531E Plasmid  AAV
AAV serotypes 1 and 6 share 99% homology in that only a 6 amino acid difference separates the two.&nbsp; Researchers were able to individually change these amino acids to represent the residue expressed by the other serotype and vice versa.&nbsp; The amino acids and their changes are listed below for this plasmid and include the parental residue, position of the residue, and residue changed to.<br /><br />AAV6.3-K531E 
 
AAV6.4-L584F Plasmid  AAV
AAV serotypes 1 and 6 share 99% homology in that only a 6 amino acid difference separates the two.&nbsp; Researchers were able to individually change these amino acids to represent the residue expressed by the other serotype and vice versa.&nbsp; The amino acids and their changes are listed below for this plasmid and include the parental residue, position of the residue, and residue changed to.<br /><br />AAV6.4-L584F 
 
AAV6.5-V598A Plasmid  AAV
AAV serotypes 1 and 6 share 99% homology in that only a 6 amino acid difference separates the two.&nbsp; Researchers were able to individually change these amino acids to represent the residue expressed by the other serotype and vice versa.&nbsp; The amino acids and their changes are listed below for this plasmid and include the parental residue, position of the residue, and residue changed to.<br /><br />AAV6.5-V598A 
 
AAV6.6-H642N Plasmid  AAV
AAV serotypes 1 and 6 share 99% homology in that only a 6 amino acid difference separates the two.&nbsp; Researchers were able to individually change these amino acids to represent the residue expressed by the other serotype and vice versa.&nbsp; The amino acids and their changes are listed below for this plasmid and include the parental residue, position of the residue, and residue changed to.<br /><br />AAV6.6-H642N 
 
AB11 Plasmid  AAV
Contains an AAV2 ITR flanked gene which encodes for CMV driven &beta;-galactocidase (Lac-Z)  
 
ACG2 Plasmid  AAV
This plasmid contains the AAV capsid and replications genes but has an ATG to ACG mutation in the replication gene Rep68/78 start site, reducing its production. 
 
Ad-hCMV-eGFP Vector  Adenovirus
This replication-defective, first generation adenoviral vector is based on human adenovirus serotype 5, with deletions in its E1a and E3 viral encoding regions. This vector encodes the transgene for enhanced green fluorescent protein (eGFP) under the control of the human cytomegalovirus (hCMV) promoter. The eGFP transgene was subcloned into the first generation adenoviral shuttle plasmid pAL119 containing the hCMV promoter to make the plasmid pAL119-eGFP. The adenoviral vector genome plasmid pJM17 (Microbix) and pAL119-eGFP were co-transfected in 293 cells (Microbix) and the adenoviral vector Ad-hCMV-eGFP was rescued by homologous recombination. Ad-hCMV-eGFP underwent three successive rounds of plaque purification and then amplified on 293 cells. Ad-hCMV-eGFP was purified from the 293 cell lysate by cesium chloride purification followed by three rounds of dialysis in 10mM Tris, ph 8.0.<br /> 
 
Ad-hCMV-Flt3L. Vector  Adenovirus
This replication-defective, first generation adenoviral vector is a based on human adenovirus serotype 5, with deletions in its E1a and E3 viral encoding regions. This vector encodes the transgene for human soluble fms-like tyrosine kinase 3 ligand (Flt3L) under the control of the human cytomegalovirus (hCMV) promoter. The Ftl3L transgene was subcloned into the first generation adenoviral shuttle plasmid pAL119 containing the hCMV promoter to make the plasmid pAL119-hsFlt3L. The adenoviral vector genome plasmid pJM17 (Microbix) and pAL119-hsFlt3L were co-transfected in 293 cells (Microbix) and the adenoviral vector Ad-hCMV-Flt3L was rescued by homologous recombination. Ad-hCMV-Flt3L underwent three successive rounds of plaque purification and then amplified on 293 cells. Ad-hCMV-Flt3L was purified from the 293 cell lysate by cesium chloride purification followed by three rounds of dialysis in 10mM Tris, ph 8.0.<br /><br /> 
 
Ad-hCMV-TK Vector  Adenovirus
This replication-defective, first generation adenoviral vector is based on human adenovirus serotype 5, with deletions in its E1a and E3 viral encoding regions. This vector encodes the transgene for Herpes Simplex Type 1 thymidine kinase under the control of the human cytomegalovirus (hCMV) promoter. The TK transgene was subcloned into the first generation adenoviral shuttle plasmid pAL119 containing the hCMV promoter to make the plasmid pAL119-TK. The adenoviral vector genome plasmid pJM17 (Microbix) and pAL119-TK were co-transfected in 293 cells (Microbix) and the adenoviral vector Ad-hCMV-TK was rescued by homologous recombination. Ad-hCMV-TK underwent three successive rounds of plaque purification and then amplified on 293 cells. Ad-hCMV-TK was purified from the 293 cell lysate by cesium chloride purification followed by three rounds of dialysis in 10mM Tris, ph 8.0.<br /> 
 
Ad-mCMV-Flt3L Vector  Adenovirus
This replication-defective, first generation adenoviral vector is based on human adenovirus serotype 5, with deletions in its E1a and E3 viral encoding regions. This vector encodes the transgene for human soluble fms-like tyrosine kinase 3 ligand (Flt3L) under the control of the murine cytomegalovirus (mCMV) promoter. The Ftl3L transgene was subcloned into the first generation adenoviral shuttle plasmid pAL120 containing the mCMV promoter to make the plasmid pAL120-hsFlt3L. The adenoviral vector genome plasmid pJM17 (Microbix) and pAL120-hsFlt3L were co-transfected in 293 cells (Microbix) and the adenoviral vector Ad-mCMV-Flt3L was rescued by homologous recombination. Ad-mCMV-Flt3L underwent three successive rounds of plaque purification and then amplified on 293 cells. Ad-mCMV-Flt3L was purified from the 293 cell lysate by cesium chloride purification followed by three rounds of dialysis in 10mM Tris, ph 8.0.<br />&nbsp;<br /> 
 
Ad-mCMV-Teton Vector  Adenovirus
This replication-defective, first generation adenoviral vector is based on human adenovirus serotype 5, with deletions in its E1a and E3 viral encoding regions. This vector encodes the transgenes for mutant tetracycline-dependent transactivator (rtTA2SM<sup>2</sup>) and transcriptional silencer carrying plasmid tTSKid under the control of the murine cytomegalovirus (mCMV) promoter. The rtTA2SM<sup>2</sup> and tTSKid are separated by an IRES sequence. The rtTA2SM<sup>2</sup>-IRES-tTSKid expression cassette was excised from the plasmid pMTIKA (Addgene plasmid 22061) and subcloned into the first generation adenoviral shuttle plasmid p&Delta;E1sp1a to make the plasmid p&Delta;E1sp1a-rtTA2SM<sup>2</sup>-IRES-tTSKid. The adenoviral vector genome plasmid pJM17 (Microbix) and p&Delta;E1sp1a-rtTA2SM<sup>2</sup>-IRES-tTSKid were co-transfected in 293 cells (Microbix) and the adenoviral vector Ad-mCMV-TetOn was rescued by homologous recombination. Ad-mCMV-TetOn underwent three successive rounds of plaque purification and then amplified on 293 cells. Ad-mCMV-TetOn was purified from the 293 cell lysate by cesium chloride purification followed by three rounds of dialysis in 10mM Tris, ph 8.0.<br /> 
 
Ad-mCMV-TK Vector  Adenovirus
This replication-defective, first generation adenoviral vector is based on human adenovirus serotype 5, with deletions in its E1a and E3 viral encoding regions. This vector encodes the transgene for Herpes Simplex Type 1 thymidine kinase under the control of the murine cytomegalovirus (mCMV) promoter. The TK transgene was subcloned into the first generation adenoviral shuttle plasmid pAL120 containing the mCMV promoter to make the plasmid pAL120-TK. The adenoviral vector genome plasmid pJM17 (Microbix) and pAL120-TK were co-transfected in 293 cells (Microbix) and the adenoviral vector Ad-mCMV-TK was rescued by homologous recombination. Ad-mCMV-TK underwent three successive rounds of plaque purification and then amplified on 293 cells. Ad-mCMV-TK was purified from the 293 cell lysate by cesium chloride purification followed by three rounds of dialysis in 10mM Tris, ph 8.0.<br /> 
 
Adenovirus Reference Standard Vector  Adenovirus
The Hex 293 cells were used in the manufacture of the Adenovirus Reference Material (ARM). The original source for the ARM is a plaque isolate from a serially plaqued sample of Adenovirus, Type 5 wild type&nbsp;(VR-5, ATCC). Plaque purification was performed on certified A549 cells (ATCC CCL-185). The plaque isolate was minimally amplified and found to be sterile, as well as free of mycoplasma and adeno-associated virus. The amplified plaque isolate was used to manufacture a Virus Bank. The adenovirus was cultured on microcarriers seeded with HEK 293 cells form a certified Working Cell Bank. The Virus Bank was certified (VR-1517) was certified to be free of adventitious agents, and was used to produce the Adenovirus Reference Material. The ARM was produced by culturing on HEK 293 cells from the Working Cell Bank in Cell Cubes&reg;, and purified using a single-column chromatography process, an anion exchange resin. The manufacture of the ARM and of the Virus Bank was performed under cGMP with full documentation. <br /><br />Recommended Use of the ARM. In the U.S., FDA-CBER has requested that sponsors use the Adenovirus Reference Material (ARM) to establish the infectious titer and particle concentration of a laboratory&#39;s internal adenovirus reference preparation, and, then, to subsequently validate sponsor particle concentration and infectious titer assays so taht units reported for adenovirus product correspond in meaning to the ARM. <br /><br />Recommended Host Cells: The ARM was prepared using a working cell bank of HEK 293 cells reserved for this project. It is not recommended that laboratories use the ARM to produce adenovirus. 
 
AmphoPhoenix Cell Line  Retrovirus
AmphoPhoenix is a vector packaging cell line containing the retroviral gag/pol genes. The vector particles are pseudotyped with the 4070A Amphotropic Virus envelope glycoprotein. Insertion of a retroviral vector construct will lead to vector production, either through transient transfection methods or through establishing a stable cell line. 
 
EcoPhoenix Cell Line  Retrovirus
EcoPhoenix is a vector packaging cell line containing the retroviral gag/pol genes. The vector particles are pseudotyped with the murine ecotropic virus envelope glycoprotein. Insertion of a retroviral vector construct will lead to vector production, either through transient transfection methods or through establishing a stable cell line. 
 
Fanconi A Vector  Retrovirus
Fanconi A Certified GMP Retroviral Vector Supernatant. 
 
Fanconi G Vector  Retrovirus
Fanconi G Certified GMP&nbsp;Retroviral Vector&nbsp;Supernatant. 
 
G156A Delta MGMT Vector  Retrovirus
Retroviral vector Master Cell Bank 
 
G1PLII Vector  Retrovirus
retroviral vector GMP Master Cell Bank 
 
GcsampL1 Vector  Retrovirus
retroviral vector Master Cell Bank 
 
GPRG Cell Line  Lentivirus
 
 
GPRG-TL20i4r-MSCV-YFP Cell Line  Lentivirus
 
 
GPRTG Cell Line  Lentivirus
GPRTG is a 293T based packaging cell line for the production of lentiviral vector particles with a VSV-G pseudotype as described in Throm, et al. The cell line expresses HIV gagpol polyprotein constitutivbely, and HIV Rev, Tat and VSV-G under the Tet-off system. 
 
HEK293 Cell Line  Adenovirus
A human cell line that can be transfected with DNA plasmids. HEK293 cells were transformecd with the Adenoviral E1A gene. 
 
HEK293T Cell Line  Lentivirus
Retrovirus
HEK293T cells are a human cell line that can be transfected with plasmid DNA at high efficiency. The cell line was derived from the HEK293 cell line by the addition of the SV40 large T antigen that has been shown to increase vector production of some viral vectors. It is commonly used for production of retroviral, lentiviral and other viral vectors using the transient transfection method. 
 
HEK293T/CD4-puro cells Cell Line  Lentivirus
Retrovirus
HEK293T/CD4 is a human cell line that can be transfected with plasmid DNA at high efficiency.&nbsp; that has been shown to increase vector productipn of some viral vectors. It is commonly used for produciton of retroviral, lentiviral and other viral vectors using the transient transfection method. The cell line has been modified to express the CD4 antigen on the cell surface, antigen is predicted to be expressed on the surface of vector particles released by the cell during vector production.&nbsp;Selected clones were further enriched by sorting. &nbsp; 
 
HSV MCB Cell Line  HSV
 
 
HyTK CML Vector  Retrovirus
retroviral vector Master Cell Bank 
 
LgCD18 Vector  Retrovirus
Retroviral Vector Master Cell Bank 
 
LhEpSNL Vector  Retrovirus
Retroviral vector Master Cell Bank 
 
MDR-1 Vector  Retrovirus
Retroviral Vector Master Cell Bank 
 
MSCV-MGMT p140K Vector  Retrovirus
Retroviral vector supernatant 
 
NIH3T3/Neo Cell Line  Retrovirus
The NIH3T3/Neo cell line is a murine cell line that contains the MSCV:hgp<sup>91</sup>pgkNeo retroviral vector expressing the gene for neomycin resistance promoted by pgk. It also expresses the human gp<sup>91</sup>-Phox gene. The line is NOT a vector producer cell line but can be used as a positive cell line for assays using drug selection for neomycin resistance (ex. G418/Geneticin&reg;). It can also be used in marker rescue assays for replication competent retroviruses. 
 
pCSCIGW Plasmid  Lentivirus
The backbone is the Addgene 3<sup>rd</sup> generation lentivirus plasmid pBOB-GFP.&nbsp; The IRES-EGFP fragment was PCR cloned and ligated into pBOB-GFP using the Age I/Xho I sites (this cloning step also removes the EGFP originally in pBOB-GFP).&nbsp; A gene of interest is inserted downstream of the CMV(min) promoter and upstream of IRES using any of the following unique sites: Nhe I, Bmt I, Afe I, Age I.&nbsp; The construct will therefore express a gene of interest and EGFP.&nbsp; Please note that the EGFP fluorescent signal will not be as robust as that seen when expressed directly from a promoter. 
 
pCSICW Plasmid  Lentivirus
The backbone is the Addgene 3<sup>rd</sup> generation lentivirus plasmid pBOB-GFP.&nbsp; The IRES-Cherry fragment was PCR cloned and ligated into pBOB-GFP using the Age I/Xho I sites (this cloning step also removes the EGFP originally in pBOB-GFP).&nbsp; A gene of interest is inserted downstream of the CMV(min) promoter and upstream of IRES using any of the following unique sites: Nhe I, Bmt I, Afe I, Age I.&nbsp; The construct will therefore express a gene of interest and mCherry.&nbsp; Please note that the mCherry fluorescent signal will not be as robust as that seen when expressed directly from a promoter. &nbsp; 
 
PG13 Cell Line  Retrovirus
PG13 is a vector packaging cell line containing the retroviral gag/pol genes. The vector particles are pseudotyped with the Gibbon Ape Leukemia Virus envelope glycoprotein. Insertion of a retroviral vector construct will lead to vector production. 
 
Phoenix GP Cell Line  Retrovirus
A variant of the Eco Ampho system the Phoenix GP expresses only gag-pol this is available for further pseudotyping of retroviral virions and other envelope proteins such as gibbon ape leukemia virus envelope or Vesicular stomatitis VSV-G protein. 
 
pLT-PGK-ble-v3 Plasmid  Lentivirus
Plasmid pLT-PGK-ble-v3 is an antibiotic resistance cassette plasmid driven by th PGK promoter and which has flanking sites for concatemerization with the lentivirus cassette in pTL20i4-MSCV-YFP 
 
pMLV-IN-Beta3ampho phoenix Vector  Retrovirus
Retroviral Vector Master Cell Bank 
 
pp65pgKNeo Vector  Retrovirus
Retroviral vector Master Cell Bank 
 
pRET2.MART-TCR Vector  Retrovirus
Retroviral Vector Master Cell Bank 
 
pSF91-GFP-WPRE Vector  Retrovirus
PSF91-GFP-WPRE is an MLV based retorviral vector with a Spleen Focus Forming Virus (SFFV) Long Terminal Repeats carrying the enhanced green Fluorescent protein gene and the woodchuck hapatitis post-transcriptional regulatory element A(WPRE). It has a truncated 5&#39; leader region as described (Hildinger 1999). It has been used as a positive control for transformaton of murine lineage-negative hematopoietic stem/progenitor cellls in the In Vitro Immortilization Assay as described (Modlich, 2006). 
 
pTL20i4-MSCV-YFP Plasmid  Lentivirus
The pTL20i4-MSCV-GFP plasmid is a lentiviral vector plasmid where the vector genome synthesis is driven by a tetracycline regulated promoter. There are restriction sites for the concatemerization of the vector genome expression cassette with the antibiotic resistance cassette from pLT-PGK-ble-v3. 
 
pTR-CBa-GFP Plasmid  AAV
 
 
pTR-CBa-Luciferase Plasmid  AAV
pTR-CBa-Luciferase - Same as pTR-CMV-Luciferase, only the CMV promoter has been replaced with the CBh (Chicken Beta Actin Short) promoter. 
 
pTR-CMV-LacZ-BGHpA Plasmid  AAV
This plasmid contains AAV serotype 2 ITRs, CMV promoter, LacZ gene and bovine growth hormone poly A. The plasmid has MCS flanking the CMV promoter, LacZ gene and BGHpA for ease of cloning. This plasmid produces a single-stranded AAV genome. 
 
pTR-CMV-Luciferase Plasmid  AAV
An AAV2 ITR containing plasmid that has a cytomegalovirus immediate early enhancer and promoter (CMV) driving the production of Luciferase. The luciferase gene floresces when the substrate Luciferine is added, alllowing the user to image viral transduction invivo without having to sacrifice an infected animal. 
 
pTRe GFP Plasmid  AAV
An AAV2 ITR containing plasmid that has a cytomegalovirus immediate early enhancer and promoter (CMV) driving the production of Green Florescent Protein (GFP) as well as the neomycin resistance driven by a SV40 promoter. 
 
pTRS-KS-CMV-GFP Plasmid  AAV
 
 
pTRS-KS/CBh-GFP Plasmid  AAV
Same as the ScAAV2-CMV-GFP plasmid, only the CMV promoter has been replaced with the CBh (Chicken Beta Actin Short) promoter allowing a slightly larger gene to be&nbsp;packaged in a self complementary vector. 
 
Purified hexon protein Antibody  Adenovirus
This purified hexon protein can be used as a standard ELISA in which the mouse anti-Adenovirus hexon Ab, Clone 8C4 (BioDesign) is used as the capture antibody and the goat anti-Adenovirus hexon-HRP conjugated (BioDesign) is used as the detection antibody. 
 
pXR 2i8 Plasmid  AAV
This plasmid is based on XR2 plasmid (contains mutations in the AAV2 CAP gene). The vector AAV2i8 is a chimeric AAV2/AAV8 vector, which is detargeted from the liver and redirected to all muscle tissues. 
 
pXR 9.45 Plasmid  AAV
This plasmid is based on the&nbsp;XR9 plasmid (contains mutations in the AAV9 CAP gene). The vector AAV9.45 is detargeted from the liver while retaining tropism for other tissues. 
 
scAAV.hFIXopt Plasmid  AAV
A self complementary ITR flanked plasmid coding for human Factor IX, which has been optimized by elimination of unfavorable <em>cis</em> -acting features that would augment hFIX expression. 
 
ScAAV2-CMV-GFP Plasmid  AAV
ScAAV2-CMV-GFP (pTRS-KS-CMV-GFP)&nbsp;&ndash; AAV packages a single strand of DNA which must undergo second strand synthesis after the DNA released from the viral capsid.&nbsp; The self complementary plasmid (Sc) contains both the possitive and negative strand of the DNA to be packaged in the vector in one plasmid, driven by the CMV (cytomegalovirus immediate early enhancer) promoter. &nbsp;This allows for the immediate expression of the Green Florescent Protein (GFP) &nbsp;vector gene resulting in faster target gene expression.&nbsp; However, the gene expressed from this vector can only be half the size of a gene that can normally be packaged by an AAV vector.  
 
sub201 Plasmid  AAV
This plasmid contains the wt AAV2 replication and capsid genes flanked by the AAV2 wt inverse terminal repeats (ITR). Essentially, this plasmid generates wtAAV2 
 
Vp2AgLuc Plasmid  AAV
Vp2AgLuc &ndash; This plasmid, built upon the ACG2 backbone, is a fusion of Gaussia Luciferase to N-terminus of Vp2.&nbsp; The usual ACG start site of this protein has been substituted for the stronger ATG start site, which allows this plasmid, when transfected along with XX6-80,&nbsp; to only make the fusion protein, no capsids. It can also be used to dope into Vp1&#43;Vp3 capsids for labeling/tracking. 
 
Vp2gfp2 Plasmid  AAV
This plasmid is the same as the&nbsp; Vp2AgLuc except that it is fused to Vp2 of AAV2 - but the fusion protein is GFP instead of gaussial luc. 
 
Vp2gluc2 Plasmid  AAV
This plasmid is the same as the Vp2AgLuc&nbsp;except that it is fused to Vp2 of AAV2. 
 
XR1 Plasmid  AAV
The XR1plasmid was constructed using the replication proteins of AAV2 found on the ACG2 plasmid and the capsid proteins of the specific serotype number in the name of the plasmid.&nbsp; When co-transfected with the XX680 and ITR containing plasmid, results in the viral vector of the serotype indicated in the name of the plasmid (ie, XR1 creates AAV serotype 1  
 
XR1-ACA Plasmid  AAV
This plasmid&nbsp;is the same as the XR plamsids of the specific serotype indicated in the name, except for a mutation in the VP2 start site resulting in the production of only VP1 and 3. 
 
XR1.1-L129F Plasmid  AAV
AAV serotypes 1 and 6 share 99% homology in that only a 6 amino acid difference separates the two.&nbsp; Researchers were able to individually change these amino acids to represent the residue expressed by the other serotype and vice versa.&nbsp; The amino acids and their changes are listed below for this plasmid and include the parental residue, position of the residue, and residue changed to.<br /><br />XR1.1-L129F 
 
XR1.2-E418D Plasmid  AAV
AAV serotypes 1 and 6 share 99% homology in that only a 6 amino acid difference separates the two.&nbsp; Researchers were able to individually change these amino acids to represent the residue expressed by the other serotype and vice versa.&nbsp; The amino acids and their changes are listed below for this plasmid and include the parental residue, position of the residue, and residue changed to.<br /><br />XR1.2-E418D 
 
XR1.3-E531K Plasmid  AAV
AAV serotypes 1 and 6 share 99% homology in that only a 6 amino acid difference separates the two.&nbsp; Researchers were able to individually change these amino acids to represent the residue expressed by the other serotype and vice versa.&nbsp; The amino acids and their changes are listed below for this plasmid and include the parental residue, position of the residue, and residue changed to.<br /><br />XR1.3-E531K 
 
XR1.4-F584L Plasmid  AAV
AAV serotypes 1 and 6 share 99% homology in that only a 6 amino acid difference separates the two.&nbsp; Researchers were able to individually change these amino acids to represent the residue expressed by the other serotype and vice versa.&nbsp; The amino acids and their changes are listed below for this plasmid and include the parental residue, position of the residue, and residue changed to.<br /><br />XR1.4-F584L 
 
XR1.5-A598V Plasmid  AAV
AAV serotypes 1 and 6 share 99% homology in that only a 6 amino acid difference separates the two.&nbsp; Researchers were able to individually change these amino acids to represent the residue expressed by the other serotype and vice versa.&nbsp; The amino acids and their changes are listed below for this plasmid and include the parental residue, position of the residue, and residue changed to.<br /><br />XR1.5-A598V 
 
XR1.6-N642H Plasmid  AAV
AAV serotypes 1 and 6 share 99% homology in that only a 6 amino acid difference separates the two.&nbsp; Researchers were able to individually change these amino acids to represent the residue expressed by the other serotype and vice versa.&nbsp; The amino acids and their changes are listed below for this plasmid and include the parental residue, position of the residue, and residue changed to.<br /><br />XR1.6-N642H 
 
XR2 Plasmid  AAV
This plasmid was constructed using the replication proteins of AAV2 found on the ACG2 plasmid and the capsid proteins of the specific serotype number in the name of the plasmid.&nbsp; When co-transfected with the XX680 and ITR containing plasmid, results in the viral vector of the serotype indicated in the name of the plasmid (i.e., XR2 creates AAV serotype&nbsp;2). 
 
XR2-ACA Plasmid  AAV
This plasmid&nbsp;is the same as the XR plamsids of the specific serotype indicated in the name, except for a mutation in the VP2 start site resulting in the production of only VP1 and 3. 
 
XR3-ACA Plasmid  AAV
This plasmid&nbsp;is the same as the XR plamsids of the specific serotype indicated in the name, except for a mutation in the VP2 start site resulting in the production of only VP1 and 3. 
 
XR3b Plasmid  AAV
This plasmid was constructed using the replication proteins of AAV2 found on the ACG2 plasmid and the capsid proteins of the specific serotype number in the name of the plasmid.&nbsp; When co-transfected with the XX680 and ITR containing plasmid, results in the viral vector of the serotype indicated in the name of the plasmid (i.e., XR3 creates AAV serotype&nbsp;3). 
 
XR4 Plasmid  AAV
This plasmid was constructed using the replication proteins of AAV2 found on the ACG2 plasmid and the capsid proteins of the specific serotype number in the name of the plasmid.&nbsp; When co-transfected with the XX680 and ITR containing plasmid, results in the viral vector of the serotype indicated in the name of the plasmid (i.e., XR4 creates AAV serotype&nbsp;4). 
 
XR4-ACA Plasmid  AAV
This plasmid&nbsp;is the same as the XR plamsids of the specific serotype indicated in the name, except for a mutation in the VP2 start site resulting in the production of only VP1 and 3. 
 
XR5-ACA Plasmid  AAV
This plasmid&nbsp;is the same as the XR plamsids of the specific serotype indicated in the name, except for a mutation in the VP2 start site resulting in the production of only VP1 and 3. 
 
XR5bam Plasmid  AAV
Xr5-Bam is a chimera of AAV2 and AAV5 rep gene along with the AAV5 capsid gene. The AAV5 rep alone did not produce virus efficiently, by adding the 5&#39; end of AAV2, to the AAV5rep, the AAV5 capsids could be produced more efficiently. 
 
XR9 Plasmid  AAV
This plasmid was constructed using the replication proteins of AAV2 found on the ACG2 plasmid and the capsid proteins of the specific serotype number in the name of the plasmid.&nbsp; When co-transfected with the XX680 and ITR containing plasmid, results in the viral vector of the serotype indicated in the name of the plasmid (i.e., XR9 creates AAV serotype&nbsp;9). 
 
XX2 Plasmid  AAV
One of the three necessary plasmids used in the triple transfection scheme of vector production, which supplies the capsid and replication proteins of the AAV genome, specifically serotype 2.&nbsp; The XX2 plasmid was constructed from the ACG2 plasmid, but differs due to a p5 element that was inserted downstream of the capsid gene. 
 
XX2-lacZ Plasmid  AAV
This plasmid is the same as the&nbsp;XX2, but contains both the ITR containing AAV-LacZ vector cassette and packaging genes. 
 
XX6-80 Plasmid  AAV
One of the three necessary plasmids used in the triple transfection scheme of vector production.&nbsp; This plasmid supplies the necessary adenoviral genes typically sequestered by AAV during it replication stage.&nbsp; This plasmid, however, does not contain the complete adenoviral genome necessary to cause generation of adenovirus.