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Reagent Information
Name Ad-hCMV-eGFP 
Reagent Type Vector 
Vector
Classifications
Adenovirus
Keywords
 
Cell Line Origin  
Short Description  
Long Description This replication-defective, first generation adenoviral vector is based on human adenovirus serotype 5, with deletions in its E1a and E3 viral encoding regions. This vector encodes the transgene for enhanced green fluorescent protein (eGFP) under the control of the human cytomegalovirus (hCMV) promoter. The eGFP transgene was subcloned into the first generation adenoviral shuttle plasmid pAL119 containing the hCMV promoter to make the plasmid pAL119-eGFP. The adenoviral vector genome plasmid pJM17 (Microbix) and pAL119-eGFP were co-transfected in 293 cells (Microbix) and the adenoviral vector Ad-hCMV-eGFP was rescued by homologous recombination. Ad-hCMV-eGFP underwent three successive rounds of plaque purification and then amplified on 293 cells. Ad-hCMV-eGFP was purified from the 293 cell lysate by cesium chloride purification followed by three rounds of dialysis in 10mM Tris, ph 8.0.  
Vector Map Ad-hCMV-eGFP.doc
Sequence  
Grade Research 
Depositor Cedars-Sinai Medical Center, Los Angeles, CA  
References 1. Vit JP, Ohara PT, Sundberg C, Rubi B, Maechler P, Liu C, Puntel M, Lowenstein P, Castro M, Jasmin L. Adenovector GAD65 gene delivery into the rat trigeminal ganglion produces orofacial analgesia. Molecular Pain. 2009 Aug 5;5:42. PMID: 19656360 
Biosafety The Indiana University Vector Production Facility works with this line under BL-2 containment but the level of containment required of any cell line or vector is at the discretion of the investigators Institutional Biosafety Committee that may require a different level of containment. 
Comments  
Storage  
Availability Amount shipped adequate for transformation of Bacteria