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Reagent Information
Name pCSCIGW 
Reagent Type Plasmid 
Vector
Classifications
Lentivirus
Keywords
 
Cell Line Origin  
Short Description  
Long Description The backbone is the Addgene 3 rd generation lentivirus plasmid pBOB-GFP. The IRES-EGFP fragment was PCR cloned and ligated into pBOB-GFP using the Age I/Xho I sites (this cloning step also removes the EGFP originally in pBOB-GFP). A gene of interest is inserted downstream of the CMV(min) promoter and upstream of IRES using any of the following unique sites: Nhe I, Bmt I, Afe I, Age I. The construct will therefore express a gene of interest and EGFP. Please note that the EGFP fluorescent signal will not be as robust as that seen when expressed directly from a promoter. 
Vector Map pCSCIGWmap.doc
Sequence pCSCIGW.txt
Grade Research 
Depositor Indiana University 
References  
Biosafety BL2 
Comments  
Storage -20C 
Availability Amount shipped adequate for transformation of Bacteria