NGVB Logo

Reagent


Add to Cart
Reagent Information
Name ScAAV2-CMV-GFP 
Reagent Type Plasmid 
Vector
Classifications
AAV
Keywords
 
Cell Line Origin  
Short Description  
Long Description ScAAV2-CMV-GFP (pTRS-KS-CMV-GFP) – AAV packages a single strand of DNA which must undergo second strand synthesis after the DNA released from the viral capsid. The self complementary plasmid (Sc) contains both the possitive and negative strand of the DNA to be packaged in the vector in one plasmid, driven by the CMV (cytomegalovirus immediate early enhancer) promoter. This allows for the immediate expression of the Green Florescent Protein (GFP) vector gene resulting in faster target gene expression. However, the gene expressed from this vector can only be half the size of a gene that can normally be packaged by an AAV vector.  
Vector Map SCAAV2-CMV-GFP.doc
Sequence SCAAV2-CMV-GFP.1.doc
Grade  
Depositor University of North Carolina Dr. Samulski  
References  
Biosafety The Indiana University Vector Production Facility works with this line under BL-2 containment but the level of containment required of any cell line or vector is at the descretion of the investigator's Institutional Biosafety Committee that may require a different level of containment. 
Comments  
Storage  
Availability