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Reagent Information
Name PG13 
Reagent Type Cell Line 
Vector
Classifications
Retrovirus
Keywords
 
Cell Line Origin Murine NIH3T3 cells 
Short Description  
Long Description PG13 is a vector packaging cell line containing the retroviral gag/pol genes. The vector particles are pseudotyped with the Gibbon Ape Leukemia Virus envelope glycoprotein. Insertion of a retroviral vector construct will lead to vector production. 
Vector Map  
Sequence  
Grade Suitable for use in Phase I clinical trials 
Depositor The PG13 cell line was created at the University of Washington in the laboratory of Dr. Dusty Miller. 
References PG13: Miller, A.D., Garcia, J.V., Von Suhr, N., Lynch, C.M. Wilson, C., and Eiden, M.V. (1991). Construciton and Properties of Retrovirus Packaging Cells based on Gibbon Ape Leukemia Virus. Journal of Virology, 65:2220-2224 
Biosafety This cell line could theoretically develop recombinations leading to the development of replication competent retroviruses, although it has failed to do so in extensive testing and clinical scale productions. The Indiana University Vector Production Facility works with this line under BL-2 containment but the level of containment required of any cell line or vector is at the descretion of the investigator's Institutional Biosafety Committee that may require a different level of containment. 
Comments As only a limited number of certified vials are available, the material will be distributed only to those investigators conducting federally funded development of clinical trial material. Grant number and specific aims should be provided to the Coordinating Center with cell line request. Individuals seeking this line for research purposes should obtain the cells through the ATCC (www.atcc.org). 
Storage  
Availability