Reagent
| Reagent Information | |
|---|---|
| Name | NIH3T3/Neo |
| Reagent Type | Cell Line |
| Vector Classifications |
Retrovirus |
| Keywords | |
| Cell Line Origin | Murine NIH3T3 cells |
| Short Description | 3T3/neo(MSCV)hgp91 |
| Long Description | The NIH3T3/Neo cell line is a murine cell line that contains the MSCV-Neo retroviral vector expressing the gene for neomycin resistance promoted by pgk. It also expresses the human gp 91 -Phox gene. The line is NOT a vector producer cell line but can be used as a positive cell line for assays using drug selection for neomycin resistance (ex. G418/Geneticin). It can also be used in marker rescue assays for replication competent retroviruses. |
| Vector Map | |
| Sequence | |
| Grade | Research |
| Depositor | The NIH3T3/Neo were created in the Indiana University Vector Production Facility |
| References | Reeves, L., Duffy, L., Koop, S., Fyffe, J., Cornetta K. Detection of ecotropic replication-competent retroviruses: Comparison of S+L- and marker rescue assays. Human Gene Therapy 13:1783-1790, 2002. |
| Biosafety | This cell line has been shown to be free of replication competent retroviruses and is of murine origin and may be suitable for work at BL-1, the level of containment required of any cell line or vector is at the dciscretion of the institutional biosafety committee that mah require a different level of containment. |
| Comments | None. |
| Storage | -80 degrees C. |
| Availability | |