Reagent
| Reagent Information | |
|---|---|
| Name | GPRTG |
| Reagent Type | Cell Line |
| Vector Classifications |
Lentivirus |
| Keywords | |
| Cell Line Origin | 293T based |
| Short Description | |
| Long Description | GPRTG is a 293T based packaging cell line for the production of lentiviral vector particles with a VSV-G pseudotype as described in Throm, et al. The cell line expresses HIV gagpol polyprotein constitutivbely, and HIV Rev, Tat and VSV-G under the Tet-off system. |
| Vector Map | |
| Sequence | |
| Grade | Research |
| Depositor | St Jude Children's Research Hospital Memphis, TN Robert E. Throm, Ph.D |
| References | Efficient construction of producer cell lines for a SIN lentiviral vector for SCID-X1 gene therapy by concatemeric array transfection. Robert E. Throm , Annastasia A. Ouma , Sheng Zhou , Anantharaman Chandrasekaran , Timothy Lockey , Michael Greene , Suk See De Ravin , Morvarid Moayeri , Harry L. Malech , Brian P. Sorrentino , John T. Gray. Blood (2009) 113 (21): 5104-5110. https://doi.org/10.1182/blood-2008-11-191049. |
| Biosafety | The Indiana Unversity Vector Production Facility works with this cell line under BL-2 containment but the level of containment required of any cell line or vector is at the discretion of the investigator's Institutional Biosafety Committee that may require a different level of containment. |
| Comments | Cell culture conditions available upon request. Tested for contaminants and mycoplasma. |
| Storage | LN2 |
| Availability | |