Reagent
| Reagent Information | |
|---|---|
| Name | pCSICW |
| Reagent Type | Plasmid |
| Vector Classifications |
Lentivirus |
| Keywords | |
| Cell Line Origin | |
| Short Description | |
| Long Description | The backbone is the Addgene 3 rd generation lentivirus plasmid pBOB-GFP. The IRES-Cherry fragment was PCR cloned and ligated into pBOB-GFP using the Age I/Xho I sites (this cloning step also removes the EGFP originally in pBOB-GFP). A gene of interest is inserted downstream of the CMV(min) promoter and upstream of IRES using any of the following unique sites: Nhe I, Bmt I, Afe I, Age I. The construct will therefore express a gene of interest and mCherry. Please note that the mCherry fluorescent signal will not be as robust as that seen when expressed directly from a promoter. |
| Vector Map | pCSICWmap.doc |
| Sequence | |
| Grade | Research |
| Depositor | Indiana University |
| References | |
| Biosafety | BL2 |
| Comments | |
| Storage | -20c |
| Availability | Amount shipped adequate for transformation of Bacteria |