Reagent
| Reagent Information | |
|---|---|
| Name | pSF91-GFP-WPRE |
| Reagent Type | Vector |
| Vector Classifications |
Retrovirus |
| Keywords | |
| Cell Line Origin | |
| Short Description | SF91GFP Vector SUP titer 2.5 x 10e6 ifu/ml |
| Long Description | PSF91-GFP-WPRE is an MLV based retorviral vector with a Spleen Focus Forming Virus (SFFV) Long Terminal Repeats carrying the enhanced green Fluorescent protein gene and the woodchuck hapatitis post-transcriptional regulatory element A(WPRE). It has a truncated 5' leader region as described (Hildinger 1999). It has been used as a positive control for transformaton of murine lineage-negative hematopoietic stem/progenitor cellls in the In Vitro Immortilization Assay as described (Modlich, 2006). |
| Vector Map | |
| Sequence | |
| Grade | GMP |
| Depositor | UCLA Donald Kohn |
| References | |
| Biosafety | BL2 |
| Comments | |
| Storage | -80 degrees C |
| Availability | |